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Derbesia sporophyte filament crush wound:

Filament is constricted and bent so cytoplasm is separated. In about one hour the filament straightens, cytoplasm in reconnected and cytoplasmic streaming recommences.

Time-lapse video microscopy of wound recovery in the siphonous green alga Derbesia tenuissima

Erica Martin, Jeremy D. Pickett-Heaps, Gwang Hoon Kim and John West

Derbesia Gametophyte Vesicle-
Cut wound:

The cut edge of the cytoplasm pulls away from the wall, showing repeated expansion and contraction. Waves of streaming occur. A new wall is formed around the smaller protoplast in about 21 hours.

Crush wound:

The cytoplasmic fragments condense, round up form new vacuoles and coalesce into a single protoplast, re-inflating to full size in 12 hours. Repeated waves of cytoplasmic streaming occur.

Time-lapse video microscopy of wound recovery in the siphonous green alga Derbesia tenuissima

Erica Martin, Jeremy D. Pickett-Heaps, Gwang Hoon Kim and John West

Derbesia Gametophyte Vesicle (3.5 mm long), puncture wound:

Side puncture, face view and apical puncture, side view.

The cytoplasm retracts and closes up at the wound site within 5 minutes. As turgor pressure increases, ruptures occur and in succession at the wound site. Cytoplasmic streaming waves are evident. The same behaviour is visible in apical puncture. The cell fully recovers in 24 hours.

Time-lapse video microscopy of wound recovery in the siphonous green alga Derbesia tenuissima

Erica Martin, Jeremy D. Pickett-Heaps, Gwang Hoon Kim and John West

Zoosporangium Formation and Discharge:

This sequence shows closure of the sporangium from the vegetative filament, activation and discharge of zoospores, zoospore with a stephanokont flagellar ring and branching at the base of the empty sporangium.

Time-lapse video microscopy of wound recovery in the siphonous green alga Derbesia tenuissima

Erica Martin, Jeremy D. Pickett-Heaps, Gwang Hoon Kim and John West
 
       
Glabrite    

Gamete Discharge:

This sequence (31 sec) shows the female gametophyte vesicle with gametangium formed, gamete activation and discharge and finally, closing of the pore and recovery of the vesicle.

Time-lapse video microscopy of wound recovery and reproduction in the siphonous green alga Derbesia tenuissima

Erica Martin, Jeremy D. Pickett-Heaps, Gwang Hoon Kim and John West

Vesicles are continually transported from the perinuclear region to the cell's exterior in the unicellular red alga Glaucosphaera vacuolata Korshikov. This phenomenon was recorded with time-lapse videomicroscopy at one frame/second and is speeded up 25x for viewing.

From Reproduction in the Red Algae

Vesicle transport and the cytoskeleton in the unicellular alga Glaucosphaera vacuolata Korshikov

Sarah M. Wilson, Jeremy D. Pickett-Heaps & John A. West
       
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